Carter then attempts to shoot the shark with a shotgun, but Susan releases it back into its pen, much to Carter's anger. However, things get out of hand when Jim ends up having his arm chopped off by the big shark. The result was a complete success as the cells start to reactivate and maintain membrane integrity in 6.5 seconds. After having it sedated and put to sleeping, Susan extracts the protein complex from its brain and applied two CCs of it onto a sample of inactive brain neurons of an Alzheimer's patient. When the day of the test is about to begin, Susan had Carter to round up the largest shark into the lab. This incident puts Aquatica's finances at risk, so Chimera Pharmaceuticals (the facility's main sponsor) sends in their board president Russell Franklin to investigate the facility.
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In the beginning of the film, one of the Mako sharks escaped captivity and attacked a boat full of teenagers, but fortunately, expert shark wrangler Carter Blake (one of the members of the team and the hero of the film) arrives to the rescue and brings the shark back to its pen. This also allows the sharks to develop an ability to swim backwards (something which a normal shark can't do) and developing a taste for other shark species, such as the time when the crew released a tiger shark into the test sharks' pen, whereupon the test sharks instantly devoured the tiger shark. As a side effect, the sharks become more smarter and dangerous than ever.
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However, it turns out that Susan herself used gene therapy to increase the brain mass, which is considered a violation of a code of ethics called the Harvard Genetics Compact.
According to one of the researchers named Janice Higgins, the team used a hormonal enhancer to increase the sharks' brain five times the normal size. This video, " Deep Blue Great White Shark Spotted Again off Coast of Hawaii", first appeared on were genetically engineered by Susan McCallister and Jim Whitlock of the Aquatica facility to produce protein complex from their brains that will serve as a cure to reactivate human brain cells. On average, female sharks range between 15-16 feet, while male sharks measure between 11-13 feet. Discovery’s 2014 feature on the great white said she measures more than 20 feet and is at least 50 years old. “We went out at sunrise, and she stayed with us pretty much throughout the day.” The Marine Conservation Science Institute later confirmed the shark to be Deep Blue. She was just big beautiful gentle giant wanting to use our boat as a scratching post,” she explained. “We saw a few tigers and then she came up and all the other sharks split, and she started brushing up against the boat. Ramsey also says she believes the shark is pregnant.
Based on its marking and size, Ramsey believed it to be “Deep Blue,” the largest great white on record and to ever be filmed. Marine biologist and conservationist Ocean Ramsey jumped in the water and started swimming with the shark.
The 20-foot-long shark approached divers off the coast of Oahu, Hawaii where they were observing tiger sharks feeding on a decomposed whale. The biggest great white shark on record was spotted again.
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